雷东升，兰州大学物理科学与技术学院教授，博士生导师，双聘兰州大学电镜中心、动物医学与生物安全学院。于2006年、2009年和2014年分别获西安交通大学理学学士、硕士和博士学位，毕业后至美国劳伦斯伯克利国家实验室工作，并于2019年06月加入兰州大学。主要发展和使用冷冻电镜三维重构技术，并将其与分子动力学模拟相结合，解析蛋白、病毒、有机框架等软凝聚态物质的三维结构，探究功能实现的分子机理，为相关疾病的药物开发、纳米材料的性能优化等提供线索。目前在Nature Chemical Biology、Nature Plants和Nature Communications等国际著名学术期刊发表论文30余篇，主持国家自然科学基金面上项目等纵横向项目，担任中国生物物理学会冷冻电子显微学分会委员、中国电子显微镜学会低温电镜专家组委员、中国生物信息学学会（筹）生物分子结构预测与模拟专业委员会委员、西北四省（甘青宁新）电子显微学会副理事长、Microstructures青年编委等职务。

1、 冷冻电镜三维重构技术及其应用。

2、 分子动力学模拟方法及其应用。

3、 蛋白、病毒、有机框架等有机大分子的动态结构与功能机理。

讲授课程

春季学期：电子显微镜及其在前沿研究中的应用（电子显微学II）、电子显微学实验方法、近代物理实验II

秋季学期：计算物理基础（含实验）II

[1] Jiang Xiong#, Shanquan Wu#, Zi-Qi Liang#, Shuo Fang, Fen-Yu Tao, Xiao-Tong Gong, Xing Wu, Qingfeng Wu, Jiao-Jiao Cui, Kun Gao, Kin Kuan Hoi, Yong Peng*, Shangwen Luo*, Dongsheng Lei*, Shi-Hui Dong*. Iterative acylation on mature lasso peptides by widespread acetyltransferases. Nature Chemical Biology, 2026, DOI:10.1038/s41589-026-02149-6

[2] Lingyun Huang#, Nanv Liu#, Weifei Chen#, Xia Ai, Haihong Li, Zelin Zhang, Ximiao Hou, Philippe Fossé, Olivier Mauffret, Dongsheng Lei*, Stephane Rety*, Xuguang Xi*. The catalytic triad of rice NARROW LEAF1 involves H234. Nature Plants, 2024, 10:743-748

[3] Shoushun Chen#*, Zelin Zhang#, Wei Chen#, Bryan E. G. Lucier, Mansheng Chen, Wanli Zhang, Haihong Zhu, Ivan Hung, Anmin Zheng, Zhehong Gan, Dongsheng Lei*, Yining Huang*. Understanding water reaction pathways to control the hydrolytic reactivity of a Zn metal-organic framework. Nature Communications, 2024, 15:10776

[4] Dongsheng Lei, Alex Marras, Jianfang Liu, Chaomin Huang, Lifeng Zhou, Shawn Zheng, Michael Braunfeld, David Agard, Carlos Castro, Hai-Jun Su*, Gang Ren*. Three-dimensional structural dynamics of DNA origami Bennett linkages using individual-particle electron tomography. Nature Communications, 2018, 9:592

[5] Lei Zhang, Dongsheng Lei, Jessica Smith, Meng Zhang, Huimin Tong, Xing Zhang, Zhuoyang Lu, Jiankang Liu, Paul Alivisatos, Gang Ren*. Three-dimensional structural dynamics and fluctuations of DNA-nanogold conjugates by individual-particle electron tomography. Nature Communications, 2016, 7:11083

[6] Shulun Huang^#, Shanquan Wu, Fengjuan Li, Pinghua Li, Pu Sun, Yimei Cao, Huifang Bao, Kaiheng Dong, Jiaxin Yang, Hehe Zhang, Qiongqiong Zhao, Ying Sun, Dong Li, Xingwen Bai, Yuanfang Fu, Hong Yuan, Xueqing Ma, Zhixun Zhao, Jing Zhang, Jian Wang, Zaixin Liu, Yong Peng, Kun Li*, Jinlian Hua*, Zengjun Lu*, Dongsheng Lei*, Qiang Zhang*. Porcine B cell receptor repertoire uncovers balanced recognition of antigenic structures on serotype Asia1 foot-and-mouth disease virus. PLoS Pathogens, 2026, 22(2):e1013893

[7] Benge Xin#, Lingyun Huang#, Linggang Yuan#, Nanv Liu, Haihong Li, Xia Ai, Dongsheng Lei, Ximiao Hou, Stephane Rety*, Xuguang Xi*. Structural insights into the N-terminal APHB domain of HrpA: mediating canonical and i-motif recognition. Nucleic Acids Research, 2024, 52(6):3406-3418

[8] Yimei Cao, Huifang Bao, Pu Sun, Xingwen Bai, Yong He, Yuanfang Fu, Hong Yuan, Xueqing Ma, Zhixun Zhao, Jing Zhang, Jian Wang, Tao Wang, Dong Li, Qiang Zhang, Jijun He, Zaixin Liu*, Zengjun Lu*, Dongsheng Lei*, Kun Li*. Discovery, recognized antigenic structures, and evolution of cross-serotype broadly neutralizing antibodies from porcine B-cell repertoires against foot-and-mouth disease virus. PLoS Pathogens, 2024, 20(10):e1012623

[9] Mengqi Jin, Zelin Zhang, Zhijiang Yu, Wei Chen, Xiaolei Wang, Dongsheng Lei*, Wenhua Zhang*. Structure–function analysis of an ancient TsaD–TsaC–SUA5–TcdA modular enzyme reveals a prototype of tRNA t6A and ct6A synthetases. Nucleic Acids Research, 2023, 51(16):8711-8729

[10] Zhixiong Zhang#, Guan Wang#, Yuyan Li#, Dongsheng Lei, Jin Xiang, Liang Ouyang, Yanyan Wang*, Jinliang Yang*. Recent progress in DNA methyltransferase inhibitors as anticancer agents. Frontiers in Pharmacology, 2022, 13:1072651

[11] Wenting Li#, Lun Tan#, Zhixiong Zhang, Qiuqi Xia, Dongsheng Lei, Yuyan Li, Ting Zhang, Shaoxue Zeng, Xiutian Sima*, Yanyan Wang*. The X-ray structure of tubulysin analogue TGL in complex with tubulin and three possible routes for the development of next-generation tubulysin analogues. Biochemical and Biophysical Research Communications. 2021, 565:29-35

[12] Xiaobo Zhai#, Dongsheng Lei#, Meng Zhang, Jianfang Liu, Hao Wu, Yadong Yu, Lei Zhang, Gang Ren*. LoTToR: An Algorithm for Missing-Wedge Correction of the Low-Tilt Tomographic 3D Reconstruction of a Single-Molecule Structure. Scientific Reports, 2020, 10:10489

[13] Xia Deng#, Hong Zhang#, Junwei Zhang, Dongsheng Lei, Yong Peng*. Synergistic effect of hierarchical nanopores in Co-doped cobalt oxide 3D flowers for electrochemical energy storage. RSC Advances, 2020, 10:43825

[14] Dongsheng Lei#, Yadong Yu#, Yu-Lin Kuang, Jianfang Liu, Ronald M. Krauss, Gang Ren*. Single-Molecule 3D Imaging of Human Plasma Intermediate-Density Lipoproteins Reveals a Polyhedral Structure. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2019, 1864(3):260-270

[15] Dongsheng Lei, Jianfang Liu, Hongbin Liu, Thomas E. Cleveland IV, John P. Marino, Ming Lei*, Gang Ren*. Single-Molecule 3D Images of "Hole-Hole" IgG1 Homodimers by Individual-Particle Electron Tomography. Scientific Reports, 2019, 9:8864

[16] Valentina Masciotti*, Luca Piantanida, Denys Naumenko, Heinz Amenitsch, Mattia Fanetti, Matjaž Valant, Dongsheng Lei, Gang Ren, Marco Lazzarino*. A DNA origami plasmonic sensor with environment-independent read-out. Nano Research, 2019, 12(11): 2900-2907

[17] Hao Wu*, Xiaobo Zhai, Dongsheng Lei, Jianfang Liu, Yadong Yu, Rongfang Bie*, Gang Ren*. An Algorithm for Enhancing the Image Contrast of Electron Tomography. Scientific Reports, 2018, 8:16711

[18] Jianfang Liu; Hao Wu; Changyu Huang; Dongsheng Lei; Meng Zhang; Wei Xie; Jinping Li; Gang Ren. Optimized Negative-Staining Protocol for Lipid–Protein Interactions Investigated by Electron Microscopy. In: Kleinschmidt J. (eds) Lipid-Protein Interactions. Methods in Molecular Biology, vol 2003. Humana, New York, NY.

[19] Meng Zhang, Dongsheng Lei, Bo Peng, Mickey Yang, M Art Charles, Kerry-Anne Rye, Ronald M Krauss, Douglas G Johns, Gang Ren*. Assessing the mechanisms of cholesteryl ester transfer protein inhibitors. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2017, 1862(12): 1606-1617

[20] Hui-Min Zhang*, Charlene Li, Ming Lei, Victor Lundin, Ho Young Lee, Milady Ninonuevo, Kevin Lin, Guanghui Han, Wendy Sandoval, Dongsheng Lei, Gang Ren, Jennifer Zhang, Hongbin Liu*. Structural and Functional Characterization of a Hole-Hole Homodimer Variant in a “Knob-Into-Hole” Bispecific Antibody. Analytical Chemistry, 2017, 89(24): 13494-13501

[21] Dongsheng Lei, Matthew Rames, Xing Zhang, Lei Zhang, Shengli Zhang*, Gang Ren*. Insights into the tunnel mechanism of cholesteryl ester transfer protein using all-atom molecular dynamics simulations. The Journal of Biological Chemistry, 2016, 291(27):14034-14044

[22] Yadong Yu, Yu-Lin Kuang, Dongsheng Lei, Xiaobo Zhai, Meng Zhang, Ronald M. Krauss, Gang Ren*. Polyhedral 3D Structure of Human Plasma Very-Low-Density Lipoproteins by Individual Particle Cryo-Electron Tomography. Journal of Lipid Research, 2016, 57(10):1879-1888

[23] Xiangyu Deng, Xiangjing Qin, Lei Chen, Qian Jia, Yonghui Zhang, Zhiyong Zhang, Dongsheng Lei, Gang Ren, Zhihong Zhou, Zhong Wang, Qing Li, Wei Xie*. Large Conformational Changes of Insertion 3 in Human Glycyl-tRNA synthetase (hGlyRS) during Catalysis. The Journal of Biological Chemistry, 2016, 291(11):5740-5752

[24] Xing Zhang, Dongsheng Lei, Lei Zhang, Matthew Rames, Shengli Zhang*. A Model of Lipid-Free Apolipoprotein AI Revealed by Iterative Molecular Dynamics Simulation. PLoS one, 2015, 10(3):e0120233

[25] Dongsheng Lei, Huimin Tong, Lei Zhang, Xing Zhang, Shengli Zhang*, Gang Ren*. Structure and Function of Cholesteryl Ester Transfer Protein in Transferring Cholesteryl Ester. Progress in Chemistry, 2014, 26(5):879-888

[26] Dongsheng Lei, Xing Zhang, Shengbo Jiang, Zhaodi Cai, Matthew J Rames, Lei Zhang, Gang Ren*, Shengli Zhang*. Structural features of cholesteryl ester transfer protein: A molecular dynamics simulation study. Proteins-Structure Function and Bioinformatics, 2013, 81(3): 415-425

[27] Lei Zhang#, Feng Yan#, Shengli Zhang, Dongsheng Lei, Arthur M. Charles, Giorgio Cavigiolio, Michael Oda, Ronald M. Krauss, Karl H. Weisgraber, Kerry-Anne Rye, Henry J Pownall, Xiayang Qiu, Gang Ren*. Structural basis of transfer between lipoproteins by cholesteryl ester transfer protein. Nature Chemical Biology, 2012, 8(4):342-349

[28] Lei Zhang, Shengli Zhang*, Huimin Tong, Dongsheng Lei, Bambi Hu. Spontaneously periodic wave generation in coupled excitable media. Physical Review E, 2009, 79(5):056213

主持国家自然科学基金面上项目1项，主持或参与甘肃省重点研发计划等多个纵横向项目。

2023    优秀报告奖             2023年全国电子显微学学术年会

2020    全国总决赛优秀奖   第七届全国青年科普创新实验暨作品大赛未来教育大学组

2020    甘肃赛区一等奖      第七届全国青年科普创新实验暨作品大赛未来教育大学组

2019    优秀报告奖             2019年全国电子显微学学术年会-生命科学显微成像技术

2019    优秀论文奖             2019年全国电子显微学学术年会

1、 中国生物物理学会冷冻电子显微学分会，委员

2、 中国电子显微镜学会低温电镜专家组，委员

3、 中国生物信息学学会（筹）生物分子结构预测与模拟专业委员会，委员

4、 中国生物物理学会人工智能生物学分会，委员

5、 西北四省（甘青宁新）电子显微镜学会，副理事长

6、 Microstructures等期刊青年编委

7、 Cell Reports、Physical Chemistry Chemical Physics、Pattern Recognition、Microstructures、Plant Physiology and Biochemistry、Molecules等期刊审稿人

手机/微信：13519133534