雷东升，男，兰州大学物理科学与技术学院教授，博士生导师，兼任西北四省（甘青宁新）电子显微学会副理事长。于2006年、2009年和2014年分别获西安交通大学理学学士、硕士和博士学位。2014年至2019年间在美国劳伦斯伯克利国家实验室工作。主要发展和使用冷冻电镜三维重构技术，并将其与分子动力学模拟相结合，解析病毒-抗体复合物、脂蛋白、框架核酸等生物大分子的三维结构，探究功能实现的分子机理，为相关疾病的药物开发等提供线索。目前在Nature Communications、Nature Plants、Nature Chemical Biology和Nucleic Acids Research等SCI刊物上发表论文20余篇，研究成果被美国能源部等20余家机构报道。主持国家自然科学基金面上项目1项，参与多项甘肃省重点研发计划或中央高校交叉创新团队项目。

1、 冷冻电镜三维重构技术及其应用； 2、 分子动力学模拟方法及其应用； 3、 病毒、脂蛋白和框架核酸等生物大分子的动态结构与功能机理。

讲授课程 秋季学期：计算物理基础实验Ⅱ（实践课） 春季学期：电子显微镜及其在前沿研究中的应用（电子显微学II）、电子显微学实验方法、计算物理基础III

[1] Lingyun Huang#, Nanv Liu#, Weifei Chen#, Xia Ai, Haihong Li, Zelin Zhang, Ximiao Hou, Philippe Fossé, Olivier Mauffret, Dongsheng Lei*, Stephane Rety*, Xuguang Xi*. The catalytic triad of rice NARROW LEAF1 involves H234. Nature Plants, 2024, doi:10.1038/s41477-024-01668-1 [2] Benge Xin#, Lingyun Huang#, Linggang Yuan#, Nanv Liu, Haihong Li, Xia Ai, Dongsheng Lei, Ximiao Hou, Stephane Rety*, Xuguang Xi*. Structural insights into the N-terminal APHB domain of HrpA: mediating canonical and i-motif recognition. Nucleic Acids Research, 2024, doi:10.1093/nar/gkae138 [3] Mengqi Jin, Zelin Zhang, Zhijiang Yu, Wei Chen, Xiaolei Wang, Dongsheng Lei*, Wenhua Zhang*. Structure–function analysis of an ancient TsaD–TsaC–SUA5–TcdA modular enzyme reveals a prototype of tRNA t6A and ct6A synthetases. Nucleic Acids Research, 2023, 51(16):8711-8729 [4] Zhixiong Zhang#, Guan Wang#, Yuyan Li#, Dongsheng Lei, Jin Xiang, Liang Ouyang, Yanyan Wang*, Jinliang Yang*. Recent progress in DNA methyltransferase inhibitors as anticancer agents. Frontiers in Pharmacology, 2022, 13:1072651. [5] Dongsheng Lei, Alex Marras, Jianfang Liu, Chaomin Huang, Lifeng Zhou, Shawn Zheng, Michael Braunfeld, David Agard, Carlos Castro, Hai-Jun Su*, Gang Ren*. Three-dimensional structural dynamics of DNA origami Bennett linkages using individual-particle electron tomography. Nature Communications, 2018, 9:592 [6] Wenting Li#, Lun Tan#, Zhixiong Zhang, Qiuqi Xia, Dongsheng Lei, Yuyan Li, Ting Zhang, Shaoxue Zeng, Xiutian Sima*, Yanyan Wang*. The X-ray structure of tubulysin analogue TGL in complex with tubulin and three possible routes for the development of next-generation tubulysin analogues. Biochemical and Biophysical Research Communications. 2021, 565:29-35 [7] Xiaobo Zhai#, Dongsheng Lei#, Meng Zhang, Jianfang Liu, Hao Wu, Yadong Yu, Lei Zhang, Gang Ren*. LoTToR: An Algorithm for Missing-Wedge Correction of the Low-Tilt Tomographic 3D Reconstruction of a Single-Molecule Structure. Scientific Reports, 2020, 10:10489 [8] Xia Deng#, Hong Zhang#, Junwei Zhang, Dongsheng Lei, Yong Peng*. Synergistic effect of hierarchical nanopores in Co-doped cobalt oxide 3D flowers for electrochemical energy storage. RSC Advances, 2020, 10:43825 [9] Dongsheng Lei#, Yadong Yu#, Yu-Lin Kuang, Jianfang Liu, Ronald M. Krauss, Gang Ren*. Single-Molecule 3D Imaging of Human Plasma Intermediate-Density Lipoproteins Reveals a Polyhedral Structure. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2019, 1864(3):260-270 [10] Dongsheng Lei, Jianfang Liu, Hongbin Liu, Thomas E. Cleveland IV, John P. Marino, Ming Lei*, Gang Ren*. Single-Molecule 3D Images of "Hole-Hole" IgG1 Homodimers by Individual-Particle Electron Tomography. Scientific Reports, 2019, 9:8864 [11] Valentina Masciotti*, Luca Piantanida, Denys Naumenko, Heinz Amenitsch, Mattia Fanetti, Matjaž Valant, Dongsheng Lei, Gang Ren, Marco Lazzarino*. A DNA origami plasmonic sensor with environment-independent read-out. Nano Research, 2019, 12(11): 2900–2907 [12] Hao Wu*, Xiaobo Zhai, Dongsheng Lei, Jianfang Liu, Yadong Yu, Rongfang Bie*, Gang Ren*. An Algorithm for Enhancing the Image Contrast of Electron Tomography. Scientific Reports, 2018, 8:16711 [13] Jianfang Liu; Hao Wu; Changyu Huang; Dongsheng Lei; Meng Zhang; Wei Xie; Jinping Li; Gang Ren. Optimized Negative-Staining Protocol for Lipid–Protein Interactions Investigated by Electron Microscopy. In: Kleinschmidt J. (eds) Lipid-Protein Interactions. Methods in Molecular Biology, vol 2003. Humana, New York, NY. [14] Meng Zhang, Dongsheng Lei, Bo Peng, Mickey Yang, M Art Charles, Kerry-Anne Rye, Ronald M Krauss, Douglas G Johns, Gang Ren*. Assessing the mechanisms of cholesteryl ester transfer protein inhibitors. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2017, 1862(12): 1606-1617 [15] Hui-Min Zhang*, Charlene Li, Ming Lei, Victor Lundin, Ho Young Lee, Milady Ninonuevo, Kevin Lin, Guanghui Han, Wendy Sandoval, Dongsheng Lei, Gang Ren, Jennifer Zhang, Hongbin Liu*. Structural and Functional Characterization of a Hole-Hole Homodimer Variant in a “Knob-Into-Hole” Bispecific Antibody. Analytical Chemistry, 2017, 89(24): 13494-13501 [16] Dongsheng Lei, Matthew Rames, Xing Zhang, Lei Zhang, Shengli Zhang*, Gang Ren*. Insights into the tunnel mechanism of cholesteryl ester transfer protein using all-atom molecular dynamics simulations. The Journal of Biological Chemistry, 2016, 291(27):14034-14044 [17] Lei Zhang, Dongsheng Lei, Jessica Smith, Meng Zhang, Huimin Tong, Xing Zhang, Zhuoyang Lu, Jiankang Liu, Paul Alivisatos, Gang Ren*. Three-dimensional structural dynamics and fluctuations of DNA-nanogold conjugates by individual-particle electron tomography. Nature Communications, 2016, 7: 11083 [18] Yadong Yu, Yu-Lin Kuang, Dongsheng Lei, Xiaobo Zhai, Meng Zhang, Ronald M. Krauss, Gang Ren*. Polyhedral 3D Structure of Human Plasma Very-Low-Density Lipoproteins by Individual Particle Cryo-Electron Tomography. Journal of Lipid Research, 2016, 57(10):1879-1888 [19] Xiangyu Deng, Xiangjing Qin, Lei Chen, Qian Jia, Yonghui Zhang, Zhiyong Zhang, Dongsheng Lei, Gang Ren, Zhihong Zhou, Zhong Wang, Qing Li, Wei Xie*. Large Conformational Changes of Insertion 3 in Human Glycyl-tRNA synthetase (hGlyRS) during Catalysis. The Journal of Biological Chemistry, 2016, 291(11):5740-5752 [20] Xing Zhang, Dongsheng Lei, Lei Zhang, Matthew Rames, Shengli Zhang*. A Model of Lipid-Free Apolipoprotein AI Revealed by Iterative Molecular Dynamics Simulation. PloS one, 2015, 10(3):e0120233 [21] Dongsheng Lei, Huimin Tong, Lei Zhang, Xing Zhang, Shengli Zhang*, Gang Ren*. Structure and Function of Cholesteryl Ester Transfer Protein in Transferring Cholesteryl Ester. Progress in Chemistry, 2014, 26(5):879-888 [22] Dongsheng Lei, Xing Zhang, Shengbo Jiang, Zhaodi Cai, Matthew J Rames, Lei Zhang, Gang Ren*, Shengli Zhang*. Structural features of cholesteryl ester transfer protein: A molecular dynamics simulation study. Proteins-Structure Function and Bioinformatics, 2013, 81(3): 415-425 [23] Lei Zhang#, Feng Yan#, Shengli Zhang, Dongsheng Lei, Arthur M. Charles, Giorgio Cavigiolio, Michael Oda, Ronald M. Krauss, Karl H. Weisgraber, Kerry-Anne Rye, Henry J Pownall, Xiayang Qiu, Gang Ren*. Structural basis of transfer between lipoproteins by cholesteryl ester transfer protein. Nature Chemical Biology, 2012, 8(4):342-349 [24] Lei Zhang, Shengli Zhang*, Huimin Tong, Dongsheng Lei, Bambi Hu. Spontaneously periodic wave generation in coupled excitable media. Physical Review E, 2009, 79(5):056213

主持国家自然科学基金面上项目1项，参与多项甘肃省重点研发计划或中央高校交叉创新团队项目。

2023 优秀报告奖　　 　2023年全国电子显微学学术年会-低温电子显微学表征分会 2020 全国总决赛优秀奖 第七届全国青年科普创新实验暨作品大赛未来教育大学组 2020 甘肃赛区一等奖　 第七届全国青年科普创新实验暨作品大赛未来教育大学组 2019 优秀报告奖　　 　2019年全国电子显微学学术年会-生命科学显微成像技术 2019 优秀论文奖　　 　2019年全国电子显微学学术年会

1、 西北四省（甘青宁新）电子显微镜学会，副理事长； 2、 中国电子显微镜学会第十一届低温电镜专家组，委员； 3、 中国生物物理学会，会员； 4、 中国生物物理学会人工智能生物学分会（筹），委员； 5、 中国生物信息学学会（筹）生物分子结构预测与模拟专业委员会，委员； 6、 Microstructures, 青年编委； 7、 Physical Chemistry Chemical Physics、Pattern Recognition、International Journal of Molecular Sciences、Molecules等期刊审稿人。

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