Dr. Dongsheng Lei received his Ph.D. degree in June 2014 from Xi’an Jiaotong University. He performed postdoctoral research at The Molecular Foundry, Lawrence Berkeley National Laboratory from 2014 to 2019, and joined the school of Physical Science and Technology, Electron Microscopy Center at Lanzhou University as a professor in 2019. He is a standing director of Electron Microscope Society of Northwestern China. He is interested in developing cryo-electron tomography technique, and combining the technique with molecular dynamics simulation to study the structure and function of macrobiomolecule. He has published 16 SCI papers, including Nature Communications, Nature Chemical Biology, Journal of Biological Chemistry. Dr. Dongsheng’s work has been highlighted and re-reported by a number of academic websites including PhysOrg.com, Nanotechnology Now, and Science Daily.

1. Methodology development for cryo-electron tomography. 2. Molecular dynamics simulation and drug discovery. 3. Structure and function of biomacromolecules.

1. Dongsheng Lei#, Yadong Yu#, Yu-Lin Kuang, Jianfang Liu, Ronald M. Krauss, Gang Ren*. Single-Molecule 3D Imaging of Human Plasma Intermediate-Density Lipoproteins Reveals a Polyhedral Structure. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2019, 1864(3):260-270. 2. Dongsheng Lei, Jianfang Liu, Hongbin Liu, Thomas E. Cleveland IV, John P. Marino, Ming Lei*, Gang Ren*. Single-Molecule 3D Images of “Hole-Hole” IgG1 Homodimers by Individual-Particle Electron Tomography. Scientific Reports, 2019, 9:8864. 3. Valentina Masciotti*, Luca Piantanida, Denys Naumenko, Heinz Amenitsch, Mattia Fanetti, Matjaž Valant, Dongsheng Lei, Gang Ren, Marco Lazzarino*. A DNA origami plasmonic sensor with environment-independent read-out. Nano Research, 2019, 12(11):2900. 4. Hao Wu*, Xiaobo Zhai, Dongsheng Lei, Jianfang Liu, Yadong Yu, Rongfang Bie*, Gang Ren*. An Algorithm for Enhancing the Image Contrast of Electron Tomography. Scientific Reports, 2018, 8:16711. 5. Dongsheng Lei, Alex Marras, Jianfang Liu, Chaomin Huang, Lifeng Zhou, Shawn Zheng, Michael Braunfeld, David Agard, Carlos Castro, Hai-Jun Su*, Gang Ren*. Three-dimensional structural dynamics of DNA origami Bennett linkages using individual-particle electron tomography. Nature Communications, 2018, 9:592. 6. Meng Zhang, Dongsheng Lei, Bo Peng, Mickey Yang, M Art Charles, Kerry-Anne Rye, Ronald M Krauss, Douglas G Johns, Gang Ren*. Assessing the mechanisms of cholesteryl ester transfer protein inhibitors. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2017, 1862(12): 1606-1617. 7. Hui-Min Zhang, Charlene Li, Ming Lei, Victor Lundin, Ho Young Lee, Milady Ninonuevo, Kevin Lin, Guanghui Han, Wendy Sandoval, Dongsheng Lei, Gang Ren, Jennifer Zhang*, Hongbin Liu*. Structural and Functional Characterization of a Hole-Hole Homodimer Variant in a “Knob-Into-Hole” Bispecific Antibody. Analytical Chemistry, 2017, 89(24): 13494-13501. 8. Dongsheng Lei, Matthew Rames, Xing Zhang, Lei Zhang, Shengli Zhang*, Gang Ren*. Insights into the tunnel mechanism of cholesteryl ester transfer protein using all-atom molecular dynamics simulations. The Journal of Biological Chemistry, 2016, 291(27):14034-14044. 9. Lei Zhang, Dongsheng Lei, Jessica Smith, Meng Zhang, Huimin Tong, Xing Zhang, Zhuoyang Lu, Jiankang Liu, Paul Alivisatos, Gang Ren*. Three-dimensional structural dynamics and fluctuations of DNA-nanogold conjugates by individual-particle electron tomography. Nature Communications, 2016, 7: 11083. 10. Yadong Yu, Yu-Lin Kuang, Dongsheng Lei, Xiaobo Zhai, Meng Zhang, Ronald M. Krauss, Gang Ren*. Polyhedral 3D Structure of Human Plasma Very-Low-Density Lipoproteins by Individual Particle Cryo-Electron Tomography. Journal of Lipid Research, 2016, 57(10):1879-1888. 11. Xiangyu Deng, Xiangjing Qin, Lei Chen, Qian Jia, Yonghui Zhang, Zhiyong Zhang, Dongsheng Lei, Gang Ren, Zhihong Zhou, Zhong Wang, Qing Li, Wei Xie*. Large Conformational Changes of Insertion 3 in Human Glycyl-tRNA synthetase (hGlyRS) during Catalysis. The Journal of Biological Chemistry, 2016, 291(11):5740-5752. 12. Xing Zhang, Dongsheng Lei, Lei Zhang, Matthew Rames, Shengli Zhang*. A Model of Lipid-Free Apolipoprotein AI Revealed by Iterative Molecular Dynamics Simulation. PloS one, 2015, 10(3):e0120233. 13. Dongsheng Lei, Huimin Tong, Lei Zhang, Xing Zhang, Shengli Zhang*, Gang Ren*. Structure and Function of Cholesteryl Ester Transfer Protein in Transferring Cholesteryl Ester. Progress in Chemistry, 2014, 26(5):879-888. 14. Dongsheng Lei, Xing Zhang, Shengbo Jiang, Zhaodi Cai, Matthew J Rames, Lei Zhang, Gang Ren*, Shengli Zhang*. Structural features of cholesteryl ester transfer protein: A molecular dynamics simulation study. Proteins-Structure Function and Bioinformatics, 2013, 81(3): 415-425. 15. Lei Zhang#, Feng Yan#, Shengli Zhang, Dongsheng Lei, Arthur M. Charles, Giorgio Cavigiolio, Michael Oda, Ronald M. Krauss, Karl H. Weisgraber, Kerry-Anne Rye, Henry J Pownall, Xiayang Qiu, Gang Ren*. Structural basis of transfer between lipoproteins by cholesteryl ester transfer protein. Nature Chemical Biology, 2012, 8(4):342-349. 16. Lei Zhang, Shengli Zhang*, Huimin Tong, Dongsheng Lei, Bambi Hu. Spontaneously periodic wave generation in coupled excitable media. Physical Review E, 2009, 79(5):056213.